Tissue Clearing | binaree.com l 3D Bio Image

whole brain

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whole brain

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200623 intestine_a-sma tile-small (1)

Figure legend 1.3 mm-thick cleared mouse intestine tissue labeled with α-SMA (abcam, ab5694, 1:50) at 37˚ for 4 days, followed by Goat anti-rabbit IgG (Alexa Fluor 647) secondary antibody(1:500). Image was acquired on a Nikon A1R microscope system. Movie was constructed with IMARIS image program. The tissue clearing solution was purchased from Binaree Inc.

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210728-PDL1_PanCK_small (1)

3D Visualization 0.2 mm-thick lung cancer (H1975) tissue labeled with PCNA (1:100) and phospho-EGFR conjugated with alexa 488 (1:100) at 37˚ for 4 days, followed by Donkey Anti-Mouse IgG (Alexa Fluor ® 647) Secondary antibody for PCNA (1:200 dilution). The nuclear counter stain is DAPI (blue). PCNA (red, Abcam, ab29), phospho-EGFR conjugated with Alexa Fluor ®488 (green, Abcam, ab205827) Microscope : Nikon A1R microscope Imaging : IMARIS software.

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3D bio Image of mouse liver tissue

0.2 mm-thick mouse liver tissue labelled with TNF-alpha (1:100) and alpha-SMA (1:100) at 37˚ for 3 days, followed by goat anti-hamster IgG (Alexa Fluor ® 647-red) and by donkey anti-rabbit IgG (Alexa Fluor488-green) secondary antibody (1:200 dilution), respectively. The nuclear counter stain is DAPI (blue). 3D images were reconstructed with IMARIS software.

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Z-stacking images of cleared 3D dermal papilla spheroids

Isolated human hair follicle dermal papilla cells were placed onto spheroid induction cell culture plate (commercially available at PAMCELLTM R-600, ANK Corporation, Korea) and formed spontaneously spheroids (diameter 100 um).

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thy-1 video

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200423 intestine_tuj1(mab1195)_1

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3D Visualization of Mouse Skin by Binaree

Binaree. www.binaree.com [Tissue Clearing : 3D Visualization of Mouse Skin by Binaree] Staining Green : Cell Nuclei (DAPI, false colour) Red : Blood vessel (Lectin FSD 647) Microscope Nikon A1R Confocal Laser Scanning Microscope Microscope Nikon A1R Confocal Laser

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3D Visualization of whole mouse heart

3D Visualization whole mouse heart stained with alpha-smooth muscle actin (red, Abcam, ab5694)

whole mouse Heart

3D Visualization whole mouse heart stained with alpha-smooth muscle actin (red, Abcam, ab5694) Microscope Light sheet image Product Binaree ImmunoStainingTM kit (HRTI-001)

thy-1 image

Thy-1 brain section

Confocal image of 1-mm-thick Thy-1 brain section using Binaree Tissue Clearing™ Kit (HRTC-001)

Mouse Skin

3D Visualization of Mouse Skin Staining Green : Cell Nuclei (DAPI, false colour) Red : Blood vessel (Lectin FSD 647) Microscope Nikon A1R Confocal Laser Scanning Microscope

Mouse Brain

Confocal image of mouse brain showing GFAP (red) and blood vessels (green) using Binaree Tissue Clearing™ Kit for Immunostaining (HRTI-001)

1-mm-thick tissue of mouse hippocampus

Confocal image of 1-mm-thick tissue of mouse hippocampus stained with β3-Tubulin (green) and NeuN (red) using Binaree Tissue Clearing™ Kit for Immunostaining (HRTI-001)

Mouse cerebellum

Confocal image of 1-mm-thick tissue of mouse cerebellum using β3-Tubulin using Binaree Tissue Clearing™ Kit for Immunostaining (HRTI-001)

1-mm-thick tissue of mouse hippocampus

Confocal image of 1-mm-thick tissue of mouse hippocampus stained with β3-Tubulin (green) and NeuN (red) using Binaree Tissue Clearing™ Kit for Immunostaining (HRTI-001)

1mm thick tissue of mouse brain

Confocal image of 1-mm-thick tissue of mouse brain using TH using Binaree Tissue Clearing™ Kit for Immunostaining (HRTI-001)

Mouse kidney

Light sheet image of blood vessels and glomeruli in the kidney using Binaree Tissue Clearing™ Kit (HRTC-001)

Mouse Eye

Light sheet image of blood vessels (green) in the mouse eye using Binaree Tissue Clearing™ Kit (HRTC-001)

Thy-1 Mouse Cerebellum

Light sheet image of Thy-1 mouse cerebellum using Binaree Tissue Clearing™ Kit (HRTC-001)

Whole Brain

Confocal Image (NIKON CONFOCAL A1R+ With CF190 20x Glys lens) of the whole brain (Thy 1-YFP) using Binaree Tissue Clearing™ Kit (HRTC-001)

1-mm-thick tissue of mouse hippocampus

Confocal image of 1-mm-thick tissue of mouse hippocampus stained with GFAP (green) and nuclei (red) using Binaree Tissue Clearing™ Kit for Immunostaining (HRTI-001)

1-mm-thick tissue of mouse cerebellum

Confocal image of 1-mm-thick tissue of mouse cerebellum using Map2 using Binaree Tissue Clearing™ Kit for Immunostaining (HRTI-001)

1-mm-thick tissue of mouse spinal cord

Confocal image of 1-mm-thick tissue of mouse spinal cord stained with MAP2 (red) using Binaree Tissue Clearing™ Kit for Immunostaining (HRTI-001)

mouse small intestine

Confocal image of mouse small intestine stained with β3-Tubulin (blue green) and nuclei (violet) using Binaree Tissue Clearing™ Kit for Immunostaining (HRTI-001)